I M. PHARM / MODERN BIO-ANALYTICAL TECHNIQUES – THEORY
After a successful completion of the course the students will be able to
| Course outcome number | Course Outcomes | Cognitive level |
| CO1 | Discuss the application of LC-MS in bioactivity screening and proteomics and the basic equipment and procedures used in cell culture techniques, including cell isolation, subculture, cryopreservation, and characterization, and explain the principles and applications of cell viability assays (MTT assays) and flow cytometry. | C1 |
| CO2 | Explain the process of bioanalytical method validation in accordance with the guidelines provided by USFDA and EMEA. | C2 |
| CO3 | Apply the principles and procedures involved in the extraction of drugs and metabolites from biological matrices using various bioanalytical methods, including protein precipitation, liquid-liquid extraction, solid-phase extraction, and novel sample preparation approaches. | C3 |
| CO4 | Analyze biopharmaceutical factors that influence drug bioavailability, including dissolution and drug release testing, and discuss alternative methods of dissolution testing and transport models and analyze in-vitro assays of drug metabolites and drug-metabolizing enzymes. | C4 |
| CO5 | Examine metabolite identification using in-vitro and in-vivo approaches, including protocols and sample preparation, and evaluate the role of microsomal approaches (Rat liver microsomes and Human liver microsomes) in metabolite identification and examine the solubility of drugs using experimental methods and evaluate drug permeability through in-vitro, in-situ, and in-vivo approaches. | C5 |
| CO6 | Create illustrative materials, including charts, diagrams, and key features, to visually represent the concepts and processes involved in the following topics use of microsomal approaches, such as Rat Liver Microsomes (RLM) and Human Liver Microsomes (HLM), in metabolite identification and protein precipitation, liquid-liquid extraction, solid-phase extraction, and innovative sample preparation techniques. | C6 |
Remembering (C1), Understanding (C2), Applying (C3), Analyzing (C4), Evaluating (C5) and Creating (C6)
